Enantioselective α-Hydroxylation of 2-Arylacetic Acid Derivatives and Buspirone Catalyzed by Engineered Cytochrome P450 BM-3
نویسندگان
چکیده
منابع مشابه
Enantioselective alpha-hydroxylation of 2-arylacetic acid derivatives and buspirone catalyzed by engineered cytochrome P450 BM-3.
Here we report that an engineered microbial cytochrome P450 BM-3 (CYP102A subfamily) efficiently catalyzes the alpha-hydroxylation of phenylacetic acid esters. This P450 BM-3 variant also produces the authentic human metabolite of buspirone, R-6-hydroxybuspirone, with 99.5% ee.
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a Institut für Organische Chemie der Technischen Universität Graz, Stremayrgasse 16, A-8010 Graz, Austria. Fax: +43 316 873 8740; Tel: +43 316 873 8751; E-mail: [email protected] b Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Fax: +1 626 568 8743; Tel: +1 626 395 4162; E-mail: [email protected] c Institut für Molek...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2006
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja061261x